PENGGUNAAN IMUNITAS KERANG HIJAU (Perna viridis) SEBAGAI BIOMARKER UNTUK MENDETEKSI PENGARUH PENGASAMAN LAUT TERHADAP TOKSISITAS LOGAM Pb

Arnold Kabangnga, Khusnul Yaqin

Abstract


Penelitian ini bertujuan menganalisis pengaruh pengasaman laut terhadap toksisitas logam Pb dengan
menggunakan biomarker imunitas kerang hijau (Perna viridis). Penelitian dilaksanakan pada bulan April hingga
Mei 2017 di Laboratorium Penangkaran dan Rehabilitasi Ekosistem Laut Fakultas Ilmu Kelautan dan
Perikanan, Universitas Hasanuddin. Metode yang digunakan dalam penelitian ini adalah rancangan faktorial.
Kerang hijau dengan ukuran panjang 5-6 cm diberi perlakuan dengan paparan konsentrasi logam Pb 0 mg/l
(kontrol), 0.008 mg/l, 0.08 mg/l, 0.8 mg/l, pada kondisi pH (level asidifikasi) air media hidup yaitu 6.2, 7.7, 8.2.
Pemaparan dilakukan selama 96 jam. Hasil penelitian menunjukkan bahwa logam Pb dan pH berpengaruh
signifikan (p<0.05) terhadap hemosit kerang yaitu persentase jumlah basofil, eosinofil dan hemosit mati.


Keywords


Biomarker, Logam Pb, Pengasaman laut, Perna viridis, Hemosit.

Full Text:

PDF

References


Addadi L., Joester D., Nudelman F., & Weiner S. (2006). Mollusk shell formation: a source of new concepts for understanding biomineralization processes. Chemistry. 12:981– 987.

Ahmad K., Bhatti I.A., Iqbal M., & Iqbal Z. (2012). Removal of heavy metals (Zn, Cr, Pb, Cd, Cu and Fe) in aqueous media by calcium carbonate as an adsorbent. International Journal of Chemical and Biochemical Sciences. 2: 48-53.

Anthony K.R.N., Kline D.I., Diaz-Pulido G., & Hoegh-Guldberg S. (2008). Ocean acidification causes bleaching and productivity loss in coral reef builders. PNAS. 105(45): 17442-17446.

Beniash E., Ivanina A., Lieb N.S., Kurochkin I., & Sokolova I.M. (2010). Elevated level of carbon dioxide affects metabolism and shell formation in oysters Crassostrea virginica. Mar Ecol Prog Ser. 419: 95–108.

Berge J.A., Bjerkeng B., Pettersen O., Schaanning M.T., & Oxnevad S. (2006). Effects of increased sea water concentrations of CO on growth of the bivalve Mytilus edulis L. Chemosphere. 62:681–687.

Biagioli M., Pinton P., Scudiero R., Ragghianti M., Bucci S., & Rizzuto R. (2005). Aequorin chimeras as valuable tool in the measurement of Ca2+ concentration during cadmium injury. Toxicology. 208: 389–398.

Bibby, Widdicombe S., Parry H., Spicer J., & Pipe R. (2008). Effects of ocean acidification on the immune response of the blue mussel Mytilus edulis. Aquat Biol. 2:67-74.

Byrne M. (2012). Global change ecotoxicology: Identification of early life history bottlenecks in marine invertebrates, variable species responses and variable experimental approaches. Mar Environ. Res. 76: 3–15.

Chapman P.M. (2002). Ecological risk assessment (ERA) and hormesis. Sci Total Environ. 288: 131-140.

Cohen A.L., & Holcomb M. (2009). Why corals care about ocean acidification: uncovering the mechanism. Oceanography. 22: 118–127.

Doney S.C., Fabry V.J., Feely R.A., & Kleypas J.A. (2009). Ocean Acidification: The Other CO2 Problem. Annu Rev Mar Sci. 1: 169-192.

Eshmat M.E., Mahasri G., & Rahardja S. (2014). Analysis of Heavy Metal Content of Lead (Pb) and Cadmium (Cd) Shells on Green (Perna viridis L.) on Water District Ngemboh Gresik East Java. Jurnal Ilmiah Perikanan dan Kelautan. 6:1

Fitzer S.C., Zhu W., Tanner K.E., Phoenix V.R., Kamenos N.A., & Cusack M. (2014). Ocean Acidification Alters The Material Properties of Mytilus edulis Shells. J. Royal Society Interface, (Online), Vol. 12, No. 103, (http://rsif.royalsocietypublishing.org/, diakses 6 Januari 2015).

Han et al. (2013). Effects of Ocean Acidification on Toxicity of Heavy Metals in The Bivalve Mytilus edulis L. Synthesis and Reactivity in Inorganic, Metal-Organic, and NanoMetal Chemistry (Online), 44: 133-139, (http://www.tandfonline.com/doi/, diakses 11 November 2014).

Jury C.P., Whitehead R.F., & Szmant A.M. (2010). Effects of variations in carbonate chemistry on the calcification rates of Madracis auretenra (Madracis mirabilis sensu Wells, 1973): bicarbonate concentrations best predict calcification rates. Glob Change Biol. 16:32–44.

Marchi B., Burlando B., Panfoli I., & Viarengo A. (2000). Interference of heavy metal cations with fluorescent Ca2+ probes does not affect Ca2+ measurements in living cells. Cell Calcium. 28: 225– 231.

Menteri Negara Lingkungan Hidup R.I. (2004). Baku Mutu Air Laut untuk Biota Laut. Deputi MENLH Bidang Kebijakan dan Kelembagaan Lingkungan Hidup. Jakarta.

Monari, M., Matozzo, V., Foschi, J., Cattani, O., Serrazanetti, G.P., Marin, M.G. 2007. Effects of high temperatures on functional responses of haemocytes in the clam Chamelea gallina. Fish Shellfish Immunol. 22: 98-114.

Ries J.B., Cohen AL., & McCorkle D.C. (2009). Marine calcifiers exhibit mixed responses to CO2-induced ocean acidification. Geology. 37:1131–1134.

Shi, H.Z. 2004. Eosinophils function as antigen-presenting cells. Journal of Leukocyte Biology. 76: 520-7

Smith J.B., Dwyer S.D., & Smith L. (1989). Cadmium evokes inositol polyphosphate formation and calcium mobilization. J. Biol. Chem. 264: 7115–7118.

Solomon S., Qin D., Manning M., Chen Z., & Marquis M. (2007). Climate Change 2007: The Physical Science Basis: Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. New York: Cambridge Univ. Press.

Talmage S.C., & Gobler C.J. (2011). Effects of elevated temperature and carbon dioxide on the growth and survival of larvae and juveniles of three species of Northwest Atlantic bivalves. PLoS ONE 6(10): e26941.

Flye-Sainte-Marie, J., Soudant, P., Lambert, C., Le Goic, N., Goncalvez, M., Travers, M.A., Paillard, C., Jean, F. 2009. Variability of the hemocyte parameters of Ruditapes philippinarum in the field during an annual cycle. J. Exp. Mar. Biol. Ecol. 377: 1-11.

Yao, C.L., Somero, G.N. 2013. Thermal stress and cellular signaling processes in hemocytes of native (Mytilus californianus) and invasive (M. galloprovincialis) mussels: cell




DOI: https://doi.org/10.26618/octopus.v8i2.3144

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Arnold Kabangnga, Khusnul Yaqin

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
OCTOPUS: Jurnal Ilmu Perikanan under by Creative Commons Attribution-NoDerivatives 4.0 International License.